Microbiota and epigenetic regulation of inflammatory mediators

Autoren:Remely, Marlene; Karlic, Heidrun (Hanusch Hospital); Rebhan, Irene; Greunz, Martina; Haslberger, Alexander G.

Bacteria and bacterial derived metabolites are known to influence the host epigenetic regulation patterns such as DNA methylation and histone modifications, thus altering the expression of critical genes in pathologic processes, for example in metabolic syndrome. Fermentation end products, especially butyrate and LPS (lipopolysaccharides), the latter being cell-wall components of gram-negative bacteria, have been suggested as bioactive metabolites influencing epigenetic modifications by directly influencing enzymes catalyzing epigenetic modifications, by altering the availability of substrates, or by interactions with receptors. Thus, identification and quantification of gut microbiota via molecular based methods are of importance to address different epigenetic patterns and gene expression. We discuss methods for microbiota, epigenetic methylation, and expression analysis of our own research which will have a role in future studies.

Anzahl der Seiten:20
Publikation Serie:Methods in Pharmacology and Toxicology, 15572153,
Digital Object Identifier (DOI):http://dx.doi.org/10.1007/978-1-4939-6743-8_8
Publikationstyp:Beitrag in Buch/Sammelband
Buchtitel:Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases