Quantification of butyryl CoA:acetate CoA-transferase genes reveals different butyrate production capacity in individuals according to diet and age

Autoren:Hippe, Berit; Zwielehner, Jutta; Liszt, Kathrin; Lassl, Cornelia; Unger, Frank; Haslberger, Alexander
Abstrakt:The gastrointestinal microbiota produces short-chain fatty acids, especially butyrate, which affect colonic health, immune function and epigenetic regulation. To assess the effects of nutrition and aging on the production of butyrate, the butyryl-CoA:acetate CoA-transferase gene and population shifts of Clostridium clusters lV and XlVa, the main butyrate producers, were analysed. Faecal samples of young healthy omnivores (24 +/- 2.5 years), vegetarians (26 +/- 5 years) and elderly (86 +/- 8 years) omnivores were evaluated. Diet and lifestyle were assessed in questionnaire-based interviews. The elderly had significantly fewer copies of the butyryl-CoA:acetate CoA-transferase gene than young omnivores (P=0.014), while vegetarians showed the highest number of copies (P=0.048). The thermal denaturation of the butyryl-CoA:acetate CoA-transferase gene variant melting curve related to Roseburia/Eubacterium rectale spp. was significantly more variable in the vegetarians than in the elderly. The Clostridium cluster XIVa was more abundant in vegetarians (P=0.049) and in omnivores (P <0.01) than in the elderly group. Gastrointestinal microbiota of the elderly is characterized by decreased butyrate production capacity, reflecting increased risk of degenerative diseases. These results suggest that the butyryl-CoA:acetate CoA-transferase gene is a valuable marker for gastrointestinal microbiota function.
Sprache:Englisch
Anzahl der Seiten:6
Publikationsdatum:2011
Journaltitel:FEMS Microbiology Letters
Volume:316
Nummer:2
Seiten:130-135
Peer reviewed:true
Links:
Digital Object Identifier (DOI):http://dx.doi.org/10.1111/j.1574-6968.2010.02197.x
Bibliographische Notiz:doi: 10.1111/j.1574-6968.2010.02197.x.
Publikationstyp:Artikel
Portal:https://ucris.univie.ac.at/portal/en/publications/quantification-of-butyryl-coaacetate-coatransferase-genes-reveals-different-butyrate-production-capacity-in-individuals-according-to-diet-and-age(9a42751f-c7f9-480c-9075-56a0d07e114b).html