Abundance and diversity of microbiota in type 2 diabetes and obesity

Autoren:Remely, Marlene; Dworzak, Simone; Hippe, Berit; Zwielehner, Jutta; Aumüller, Eva; Brath, Helmut; Haslberger, Alexander
Abstrakt:Inflammatory reactions leading to the progression of metabolic syndrome contribute to changes in compositionof the GIT microbiota.We characterize fecal microbiota at three time points in fourteen obese participants, nineteen lean controls andtwenty-four type 2 diabetes patients. Obese and type 2 diabetics received an intervention of nutritional counseling,type 2 diabetics an additional therapy with a GLP-1-Agonist.The microbiota composition was analyzed for abundanceand diversity by quantitative real-time polymerase chain reaction, denaturing gradient gel electrophoresis and highthroughput sequencing.In type 2 diabetics an increase of diversity was observed with intervention whereas the values of lean controlsremained unaffected. In the lean and obesity groups, a lower Firmicutes:Bacteroidetes ratio correlated with lowerBMI. In type 2 diabetics the ratio of Firmicutes to Bacteroidetes increased throughout the intervention period. Type2 diabetics showed a significantly enhanced proportion of lactic acid bacteria before and after intervention, alsoAkkermansia and Enterobacteria showed a higher abundance in type 2 diabetics, increasing throughout the studyperiod. Archaea were significantly more frequent in type 2 diabetics.Whether differences seen in the abundance of certain groups and in the diversity of microbiota reflect differentunderlying inflammatory mechanisms of type 2 diabetes and obesity or rather the progression of the metabolicdisease remains unclear and needs long-term investigation.
Anzahl der Seiten:8
Journaltitel:Journal of Diabetes & Metabolism
Peer reviewed:true
Digital Object Identifier (DOI):http://dx.doi.org/10.4172/2155-6156.1000253