Funding Body: Internal 

Duration: 2014 - 2017

Principal Investigators:

Karl-Heinz Wagner
Helmut Brath, Diabetes Outpatient Clinic South, Vienna

Main Project group:

Annemarie Grindel, PhD studentVerena Sauermoser, Master studentBianca Gugenberger, Master studentChristina Pöppelmeyer, Master studentAmelie Kuchler, Master studentAlexandra Pieler, Master studentMichaela Gschaider, Master studentLukas Eichberger , Master studentSylvia Dörfler Master studentCordula Haidenthaler, Master studentAnnelies Aigner, Master student

Short description:

Diabetes mellitus type 2 (T2DM) is associated with oxidative stress which in turn can lead to DNA damage. The aim of the present study was to analyze oxidative stress, chromosomal damage, DNA damage and DNA repair in regard to hyperglycemic state and diabetes duration.

Female T2DM patients (n=146) were enrolled in the MIKRODIAB study and allocated in two groups regarding their glycated hemoglobin (HbA1c) level (HbA1c≤7.5 %, n=74; HbA1c>7.5 %, n=72). In addition, tertiles according to diabetes duration (DD) were created (DDI=6.94±3.1 y, n=49; DDII=13.35±1.1 y, n=48; DDIII=22.90±7.3 y, n=49). 

Primary Outcome Measures:

• The quality of HbA1c influences micronuclei formation in binucleated cells in this cross-sectional study the micronuclei formation in binucleated lymphocytes (Number of micronuclei/1000 binucleated cells) is compared between a low (HbA1c<7.5%) and a high (HbA1c>7.5%) glycemic group
• Secondary Outcome Measures: Type 2 diabetes duration of the subjects influences chromosomal damage. The micronuclei formation in binucleated lymphocytes (Number of micronuclei/1000 binucleated cells) is associated with the duration of the Typ 2 diabetes disease.

The study has been registered at ClinicalTrials.gov under the Number NCT02231736