Anti-leukaemic effects of two extract types of Lactuca sativa correlate with the activation of Chk2, induction of p21, downregulation of cyclin D1 and acetylation of alpha-tubulin

Autoren:Gridling, Manuela (Universitätsklinik, Medizinische Universität Wien); McKinnon, Ruxandra; Kopp, Brigitte; Wagner, Karl-Heinz; Krenn, Liselotte; Krupitza, Georg (Medizinische Universität Wien)
Abstrakt:The water extract of the lettuce Lactuca sativa, but not the ethyl acetate extract, inhibited the growth of HL-60 leukaemia cells and MCF-7 breast cancer cells. This correlated with the activation of checkpoint kinase 2 (Chk2). the induction of the tumour suppressor p21, and the severe downregulation of the proto-oncogene cyclin D1. The ethyl acetate extract, but not the water extract, induced HL-60 cell death, which correlated With the aectylation of alpha-tubulin. The acetylation of alpha-tubulin is indicative for microtubuli stabilisation such as induced by taxol. The calculated amount for human intake would require approximately 3 kg lettuce to reach the required concentration shown to inhibit 50% HL-60 proliferation.
Sprache:Englisch
Anzahl der Seiten:7
Publikationsdatum:2010
Journaltitel:Oncology Reports
Volume:23
Nummer:4
Seiten:1145-1151
Peer reviewed:true
Links:
Digital Object Identifier (DOI):http://dx.doi.org/10.3892/or_00000744
Bibliographische Notiz:***112548.28***
Publikationstyp:Artikel
Portal:https://ucris.univie.ac.at/portal/en/publications/antileukaemic-effects-of-two-extract-types-of-lactuca-sativa-correlate-with-the-activation-of-chk2-induction-of-p21-downregulation-of-cyclin-d1-and-acetylation-of-alphatubulin(3027c0cb-8d9a-4886-90bf-26774b2b7f44).html