Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters

Untertitel:Results of human intervention trials
Autoren:Ferk, Franziska (Medizinische Universität Wien); Mišík, Miroslav (Medizinische Universität Wien); Nersesyan, Armen (Medizinische Universität Wien); Pichler, Christoph (Medizinische Universität Wien); Jäger, Walter; Szekeres, Thomas (Medizinische Universität Wien); Marculescu, Rodrig (Medizinische Universität Wien); Poulsen, Henrik E. (Laboratory of Clinical Pharmacology Copenhagen); Henriksen, Trine (Laboratory of Clinical Pharmacology Copenhagen); Bono, Roberto (Università degli Studi di Torino); Romanazzi, Valeria (Università degli Studi di Torino); Al-Serori, Halh (Medizinische Universität Wien); Biendl, Martin (Hallertauer Hopfenveredelung GmbH); Wagner, Karl Heinz; Kundi, Michael (Medizinische Universität Wien); Knasmüller, Siegfried (Medizinische Universität Wien)

Scope: Xanthohumol (XN) is a hop flavonoid found in beers and refreshment drinks. Results of in vitro and animal studies indicate that it causes beneficial health effects due to DNA protective, anti-inflammatory, antioxidant, and phytoestrogenic properties. Aim of the present study was to find out if XN causes alterations of health-related parameters in humans. Methods and results: The effects of the flavonoid were investigated in a randomized crossover intervention trial (n = 22) in which the participants consumed a XN drink (12 mg XN/P/day). We monitored alterations of the DNA stability in single cell gel electrophoresis assays in lymphocytes and of several health-related biomarkers. A decrease of oxidatively damaged purines and protection toward reactive oxygen species induced DNA damage was found after the consumption of the beverage; also the excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-guanosine in urine was reduced. The assumption that the flavonoid causes DNA protection was confirmed in a randomized follow-up study with pure XN (n = 10) with a parallel design. Other biochemical parameters reflecting the redox- and hormonal status and lipid- and glucose metabolism were not altered after the intervention. Conclusion: Taken together, our data indicate that low doses of XN protect humans against oxidative DNA damage. The effects of xanthohumol were investigated in a crossover intervention trial in which the participants consumed a drink containing 12 mg XN/P/day. Alterations of the DNA stability were monitored in single cell gel electrophoresis assays in lymphocytes. A decrease of oxidatively damaged purines and protection toward reactive oxygen species (ROS)-induced DNA damage was found after consumption of the beverage; furthermore, also urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-guanosine were reduced.

Anzahl der Seiten:14
Journaltitel:Molecular Nutrition & Food Research
Peer reviewed:true
Digital Object Identifier (DOI):