Treatment with alpha-galactosylceramide protects mice from early onset of non-alcoholic steatohepatitis

Untertitel:role of intestinal barrier function
Autoren:Engstler, Anna Janina (Friedrich-Schiller-Universität Jena); Sellmann, Cathrin (Friedrich-Schiller-Universität Jena); Jin, Cheng Jun (Friedrich-Schiller-Universität Jena); Brandt, Annette; Herz, Kathleen (Friedrich-Schiller-Universität Jena); Priebs, Josephine (Friedrich-Schiller-Universität Jena); Bergheim, Ina (Friedrich-Schiller-Universität Jena)
Abstrakt:

SCOPE: The role of invariant natural killer T cells in the development of non-alcoholic steatohepatitis (NASH) has not yet been fully understood. Here, the effect of the invariant natural killer T cell activator alpha-galactosylceramide (αGalCer) on the development of non-alcoholic fatty liver disease and intestinal barrier function was assessed in a mouse model of early Western-style diet (WSD)-induced NASH.

METHODS AND RESULTS: Female C57BL/6J mice were either fed a liquid control diet or a liquid fructose-enriched WSD for 6 weeks while being treated 3-times weekly with αGalCer (2 μg i.p.) or vehicle. Indices of liver damage, glucose metabolism and intestinal permeability were measured. Treatment with αGalCer markedly suppressed hepatic fat accumulation and inflammation while not affecting fasting glucose. The protective effects of αGalCer were associated with a protection against the increased translocation of bacterial endotoxins and the decreased protein levels of tight junction proteins occludin and zonula occludens 1 found in vehicle-treated mice while being fed a WSD.

CONCLUSION: Taken together, our data suggest that the protective effects of αGalCer against the development of a diet-induced NASH in mice are associated with a protection against the increased translocation of intestinal bacterial endotoxins associated with the development of NASH. This article is protected by copyright. All rights reserved.

Sprache:Englisch
Anzahl der Seiten:11
Publikationsdatum:9.1.2017
Journaltitel:Molecular Nutrition & Food Research
Peer reviewed:true
Links:
Digital Object Identifier (DOI):http://dx.doi.org/10.1002/mnfr.201600985
Bibliographische Notiz:This article is protected by copyright. All rights reserved.
Publikationstyp:Artikel
Portal:https://ucris.univie.ac.at/portal/en/publications/treatment-with-alphagalactosylceramide-protects-mice-from-early-onset-of-nonalcoholic-steatohepatitis(00c4f3d0-65fe-4d80-a942-21a7686f7b20).html