Impact of a probiotic, inulin, or their combination on the piglets' microbiota at different intestinal locations

Autor(en)

V A Sattler, K Bayer, G Schatzmayr, A G Haslberger, V Klose

Abstrakt

Natural feed additives are used to maintain health and to promote performance of pigs without antibiotics. Effects of a probiotic, inulin, and their combination (synbiotic), on the microbial diversity and composition at different intestinal locations were analysed using denaturing gradient gel electrophoresis (DGGE), real-time PCR, and 16S rRNA gene pyrosequencing. Bacterial diversity assessed by DGGE and/or pyrosequencing was increased by inulin in all three gut locations and by the synbiotic in the caecum and colon. In contrast, the probiotic did only affect the microbiota diversity in the ileum. Shifts in the DGGE microbiota profiles of the caecum and colon were detected for the pro- and synbiotic fed animals, whereas inulin profiles were more similar to the ones of the control. 16S rRNA gene pyrosequencing revealed that all three additives could reduce Escherichia species in each gut location, indicating a potential beneficial effect on the gut microbiota. An increase of relative abundance of Clostridiaceae in the large intestine was found in the inulin group and of Enterococcaceae in the ileum of probiotic fed pigs. Furthermore, real-time PCR results showed that the probiotic and synbiotic increased bifidobacterial numbers in the ileum, which was supported by sequencing results. The probiotic and inulin, to different extents, changed the diversity, relative abundance of phylotypes, and community profiles of the porcine microbiota. However, alterations of the bacterial community were not uniformly between gut locations, demonstrating that functionality of feed additives is site specific. Therefore, gut sampling from various locations is crucial when investigations aim to identify the composition of a healthy gut microbiota after its manipulation through feed additives.

Organisation(en)

Department für Ernährungswissenschaften

Externe Organisation(en)

Universität für Bodenkultur Wien, BIOMIN Research Center

Journal

Beneficial Microbes

Band

6

Seiten

473-483

Anzahl der Seiten

11

ISSN

1876-2883

DOI

https://doi.org/10.3920/BM2014.0030

Publikationsdatum

01-2015

Peer-reviewed

Ja

ÖFOS 2012

303002 Bakteriologie

Schlagwörter

ASJC Scopus Sachgebiete

Microbiology (medical), Microbiology

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/impact-of-a-probiotic-inulin-or-their-combination-on-the-piglets-microbiota-at-different-intestinal-locations(562dee12-c0db-4f45-ba8d-4be810c6a4f5).html