Quantification of butyryl CoA:acetate CoA-transferase genes reveals different butyrate production capacity in individuals according to diet and age
- Autor(en)
- Berit Hippe, Jutta Zwielehner, Kathrin Liszt, Cornelia Lassl, Frank Unger, Alexander Haslberger
- Abstrakt
The gastrointestinal microbiota produces short-chain fatty acids, especially butyrate, which affect colonic health, immune function and epigenetic regulation. To assess the effects of nutrition and aging on the production of butyrate, the butyryl-CoA:acetate CoA-transferase gene and population shifts of Clostridium clusters lV and XlVa, the main butyrate producers, were analysed. Faecal samples of young healthy omnivores (24 +/- 2.5 years), vegetarians (26 +/- 5 years) and elderly (86 +/- 8 years) omnivores were evaluated. Diet and lifestyle were assessed in questionnaire-based interviews. The elderly had significantly fewer copies of the butyryl-CoA:acetate CoA-transferase gene than young omnivores (P=0.014), while vegetarians showed the highest number of copies (P=0.048). The thermal denaturation of the butyryl-CoA:acetate CoA-transferase gene variant melting curve related to Roseburia/Eubacterium rectale spp. was significantly more variable in the vegetarians than in the elderly. The Clostridium cluster XIVa was more abundant in vegetarians (P=0.049) and in omnivores (P <0.01) than in the elderly group. Gastrointestinal microbiota of the elderly is characterized by decreased butyrate production capacity, reflecting increased risk of degenerative diseases. These results suggest that the butyryl-CoA:acetate CoA-transferase gene is a valuable marker for gastrointestinal microbiota function.
- Organisation(en)
- Department für Ernährungswissenschaften, Institut für Physiologische Chemie, Fakultät für Lebenswissenschaften
- Journal
- FEMS Microbiology Letters
- Band
- 316
- Seiten
- 130-135
- Anzahl der Seiten
- 6
- ISSN
- 0378-1097
- DOI
- https://doi.org/10.1111/j.1574-6968.2010.02197.x
- Publikationsdatum
- 2011
- Peer-reviewed
- Ja
- ÖFOS 2012
- 303009 Ernährungswissenschaften
- Link zum Portal
- https://ucris.univie.ac.at/portal/de/publications/quantification-of-butyryl-coaacetate-coatransferase-genes-reveals-different-butyrate-production-capacity-in-individuals-according-to-diet-and-age(9a42751f-c7f9-480c-9075-56a0d07e114b).html