Interleukin-8 stimulates calcium transients and promotes epidermal cell proliferation

Autor(en)

A Tuschil, C Lam, A Haslberger, I Lindley

Abstrakt

The presence of large amounts of biologically active interleukin-8 (IL-8) in psoriatic involved skin suggests that it may contribute, in part, to the changes observed in psoriasis, including hyperproliferation of keratinocytes. To examine the effect of IL-8 on epidermal growth, we monitored cytosolic free Ca++ transients in human keratinocytes adult skin epidermis calcium reduced level, temperature elevated (HaCat) cells and normal keratinocytes loaded with the cell permeable, acetoxymethyl derivative, indo-1AM. Addition of IL-8 (0.06-47 nM) to the HaCat cells induced rapid rises in cytosolic free Ca++ from resting levels of 145 +/- 38 to peak levels of 889 +/- 10 nM. The induced rises in Ca++ were transient and concentration dependent. Half maximal effect was observed at 1.2 nM. Normal keratinocytes also responded to IL-8 (6 nM) by rises in cytosolic free Ca++ from a pre-stimulated level of 269 nM to transient peak value of 393 nM. In addition, IL-8 promoted epidermal cell proliferation. Polyclonal anti-IL-8 antibody blocked IL-8-induced calcium changes and proliferation. Under similar conditions, human neutrophils also responded to IL-8 in a similar dose range by a rapid and transient mobilization of Ca++. The findings indicate that IL-8 has a wider range of responsive target cells than hitherto thought and acts as an autocrine growth factor.

Organisation(en)

Department für Ernährungswissenschaften

Externe Organisation(en)

Sandoz GmbH

Journal

Journal of Investigative Dermatology

Band

99

Seiten

294-8

Anzahl der Seiten

5

ISSN

0022-202X

Publikationsdatum

09-1992

Peer-reviewed

Ja

ÖFOS 2012

301304 Medizinische Biologie

Schlagwörter

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/interleukin8-stimulates-calcium-transients-and-promotes-epidermal-cell-proliferation(acd2e7d8-fd1e-4087-b9d4-4484dc838026).html