B lymphocytes with latent EBV infection appearing in long-term bone marrow cultures (HLTBMCs) from haematological patients induce lysis of stromal microenvironment

Autor(en)
B G Pavlova, H H Mühlberger, H Strobl, R Grill, A Haslberger, F Varga, H Auer, R Heinz, J Salamon, A Stacher, Heidrun Karlic
Abstrakt

Human long-term bone marrow cultures (HLTBMCs) are a valuable in vitro model for studying the role of the haemopoietic microenvironment. Here we report the spontaneous appearance of EBV-positive B cells in 6/40 HLTBMCs from patients with various haematological diseases after 3-5 months of culture. After subcultivation of these cells, a novel type of cell line could be characterized, which displayed surface markers and morphological features typical for EBV transformed B-cell lines. As the deproteinized and ultrafiltrated culture supernatants of these cell lines were found to contain an agent with stroma toxic properties, they were termed SSB lines (stroma-toxic-agent-secreting B-cell lines). This agent also exhibited a colony-inhibitory activity on in vitro myelopoiesis and erythropoiesis. These properties are typical for the two polyamines spermine and spermidine which were detected at elevated levels in the culture supernatants of SSB lines. The hypothesis that latent presence of EBV in bone marrow may induce an increased synthesis of spermine and spermidine, which are known to be associated with malignant haematological diseases and bone marrow aplasia, is discussed.

Organisation(en)
Department für Ernährungswissenschaften
Externe Organisation(en)
Ludwig-Boltzmann-Institut für Leukämieforschung und Hämatologie, Hanusch Krankenhaus
Journal
British Journal of Haematology (BJH)
Band
89
Seiten
704-711
Anzahl der Seiten
8
ISSN
0007-1048
DOI
https://doi.org/10.1111/j.1365-2141.1995.tb08405.x
Publikationsdatum
04-1995
Peer-reviewed
Ja
ÖFOS 2012
303012 Gesundheitswissenschaften
Schlagwörter
Sustainable Development Goals
SDG 3 – Gesundheit und Wohlergehen
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/b-lymphocytes-with-latent-ebv-infection-appearing-in-longterm-bone-marrow-cultures-hltbmcs-from-haematological-patients-induce-lysis-of-stromal-microenvironment(ccbb53b7-803e-4176-a9e8-4c66dc446a8a).html