Dietary nitrate, vascular function, and inflammation

Research funding: Austrian Science Fund (FWF), funding program: Clinical Research, Project no. KLI 858

PI: Oliver Neubauer

Research institutions: University of Vienna (lead), Medical University of Vienna

International research and cooperation partners: Catherine Bondonno, Jonathan Hodgson (both Edith Cowan University, Australia)

Start: 2021; End: 2024

Link: https://www.fwf.ac.at/en/research-radar/10.55776/KLI858

Project summary:

The risk for cardiovascular diseases (CVD) increases with advancing age. Developing effective lifestyle-based strategies to promote, preserve or restore cardiovascular health with ageing is therefore a high priority. With this clinical research, we investigate the innovative concept that an increased intake of dietary nitrate (through beetroot juice) could be a feasible adjuvant therapy to treat elevated blood pressure and improve blood vessel function in older adults. High blood pressure is another major risk factor for CVD.

Inorganic dietary nitrate, found abundantly in beetroot and green leafy vegetables, is a source of nitric oxide (NO), a signalling molecule that is important for cardiovascular health. NO is also produced in the human body, but the body’s production and availability of NO decrease during ageing and CVD. The declined NO availability is associated with impaired blood vessel function, unresolved inflammatory responses, and an increased CVD risk. Dietary nitrate is an additional NO source. Following the intake of nitrate through the diet, NO is produced in a pathway that involves commensal bacteria in the mouth. Dietary nitrate elicits various beneficial physiological effects, but these benefits have mostly been shown in young, healthy individuals. As one of a few studies involving an older population group, the results of our previous study suggested positive short-term effects of nitrate-rich beetroot juice on blood pressure and inflammation markers in healthy older adults. However, little is known about whether dietary nitrate improves cardiovascular health in older populations with high blood pressure.

The overall aim of this randomized, placebo-controlled crossover study is to investigate whether the daily intake of nitrate-rich beetroot juice over four weeks translates into improved cardiovascular health-related outcomes in older adults with treated mild high blood pressure. Men and women, between the ages of 55 and 70 years, who have been diagnosed with grade 1 high blood pressure and who are taking two or more blood-pressure lowering medications will be recruited. The study will investigate whether the increased dietary nitrate intake further lowers blood pressure and improves blood vessel function. A specific aim is to examine whether the nitrate intake results in favorable changes in the oral bacteria community and the systemic inflammatory status, and whether these changes correlate with cardiovascular-related outcomes. This research will offer information on the value of dietary nitrate to counteract chronic inflammation, the latter of which plays a role in developing or worsening cardiovascular disorders, such as high blood pressure.

Collectively, the expected results of this study will provide important new evidence of whether nitrate-rich beetroot juice could be a key component of therapeutic interventions to improve cardiovascular health in individuals with high blood pressure.