PPARγ Modulates Long Chain Fatty Acid Processing in the Intestinal Epithelium

Autor(en)

Kalina Duszka, Matej Oresic, Cedric Le May, Jürgen König, Walter Wahli

Abstrakt

Nuclear receptor PPARγ affects lipid metabolism in several tissues, but its role in intestinal lipid metabolism has not been explored. As alterations have been observed in the plasma lipid profile of ad libitum fed intestinal epithelium-specific PPARγ knockout mice (iePPARγKO), we submitted these mice to lipid gavage challenges. Within hours after gavage with long chain unsaturated fatty acid (FA)-rich canola oil, the iePPARγKO mice had higher plasma free FA levels and lower gastric inhibitory polypeptide levels than their wild-type (WT) littermates, and altered expression of incretin genes and lipid metabolism-associated genes in the intestinal epithelium. Gavage with the medium chain saturated FA-rich coconut oil did not result in differences between the two genotypes. Furthermore, the iePPARγKO mice did not exhibit defective lipid uptake and stomach emptying; however, their intestinal transit was more rapid than in WT mice. When fed a canola oil-rich diet for 4.5 months, iePPARγKO mice had higher body lean mass than the WT mice. We conclude that intestinal epithelium PPARγ is activated preferentially by long chain unsaturated FAs compared to medium chain saturated FAs. Furthermore, we hypothesize that the iePPARγKO phenotype originates from altered lipid metabolism and release in epithelial cells, as well as changes in intestinal motility.

Organisation(en)

Department für Ernährungswissenschaften, Forschungsplattform Vienna Metabolomics Center

Externe Organisation(en)

University of Turku, Nanyang Technological University (NTU), Université de Nantes, Université de Lausanne, INRA - Institut national de la recherche agronomique

Journal

International Journal of Molecular Sciences

Band

18

Anzahl der Seiten

15

ISSN

1661-6596

DOI

https://doi.org/10.3390/ijms18122559

Publikationsdatum

12-2017

Peer-reviewed

Ja

ÖFOS 2012

303009 Ernährungswissenschaften

Schlagwörter

ASJC Scopus Sachgebiete

Molecular Biology, Spectroscopy, Catalysis, Inorganic Chemistry, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/ppar-modulates-long-chain-fatty-acid-processing-in-the-intestinal-epithelium(7d1ac407-a8a4-4fc4-aa84-0a2b85849684).html