Increased gut microbiota diversity and abundance of Faecalibacterium prausnitzii and Akkermansia after fasting

Autor(en)

Marlene Remely, Berit Hippe, Isabella Geretschlaeger, Sonja Stegmayer, Ingrid Hoefinger, Alexander Haslberger

Abstrakt

An impaired gut microbiota has been reported as an important factor in the pathogenesis of obesity. Weight reduction has already been mentioned to improve gut microbial subpopulations involved in inflammatory processes, though other subpopulations still need further investigation. Thus, weight reduction in the context of a fasting program together with a probiotic intervention may improve the abundance and diversity of gut microbiota.

In this pilot study, overweight people underwent a fasting program with laxative treatment for one week followed by a six week intervention with a probiotic formula. Gut microbiota were analyzed on the basis of 16s rDNA with quantitative real time polymerase chain reaction. Additionally a food frequency questionnaire with questions about nutritional behavior, lifestyle, and physical activity was administered before and after intervention.

We observed an increase in microbial diversity over the study period. No significant changes in abundance of total bacteria, or of Bacteroidetes, Prevotella, Clostridium cluster XIVa, or Clostridium cluster IV were found, although Faecalibacterium prausnitzii showed an increase over study period. In addition, Akkermanisa and Bifidobacteria increased in abundance due to intervention. The inflammation-associated gut microbes Enterobacteria and Lactobacilli increased during the first week and then declined by the end of the intervention. Two-thirds of the study participants harbored Archaea. No significant improvements of eating habits were reported, although physical activity improved due to the intervention.

Our results show that caloric restriction affects gut microbiota by proliferating mucin-degrading microbial subpopulations. An additional intervention with a probiotic formula increased probiotic-administered gut microbial populations.

Organisation(en)

Department für Ernährungswissenschaften

Externe Organisation(en)

Essen & Fasten, Universität Wien

Journal

Wiener Klinische Wochenschrift: The Central European Journal of Medicine

Band

127

Seiten

394-398

Anzahl der Seiten

5

ISSN

0043-5325

DOI

https://doi.org/10.1007/s00508-015-0755-1

Publikationsdatum

03-2015

Peer-reviewed

Ja

ÖFOS 2012

303009 Ernährungswissenschaften

Schlagwörter

ASJC Scopus Sachgebiete

Medicine(all)

Sustainable Development Goals

SDG 3 – Gesundheit und Wohlergehen

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/increased-gut-microbiota-diversity-and-abundance-of-faecalibacterium-prausnitzii-and-akkermansia-after-fasting(07cbccb2-7422-4eef-b76d-2ecac304cf37).html