Abundance and diversity of microbiota in type 2 diabetes and obesity

Autor(en)

Marlene Remely, Simone Dworzak, Berit Hippe, Jutta Zwielehner, Eva Aumüller, Helmut Brath, Alexander Haslberger

Abstrakt

Inflammatory reactions leading to the progression of metabolic syndrome contribute to changes in composition
of the GIT microbiota.
We characterize fecal microbiota at three time points in fourteen obese participants, nineteen lean controls and
twenty-four type 2 diabetes patients. Obese and type 2 diabetics received an intervention of nutritional counseling,
type 2 diabetics an additional therapy with a GLP-1-Agonist.The microbiota composition was analyzed for abundance
and diversity by quantitative real-time polymerase chain reaction, denaturing gradient gel electrophoresis and high
throughput sequencing.
In type 2 diabetics an increase of diversity was observed with intervention whereas the values of lean controls
remained unaffected. In the lean and obesity groups, a lower Firmicutes:Bacteroidetes ratio correlated with lower
BMI. In type 2 diabetics the ratio of Firmicutes to Bacteroidetes increased throughout the intervention period. Type
2 diabetics showed a significantly enhanced proportion of lactic acid bacteria before and after intervention, also
Akkermansia and Enterobacteria showed a higher abundance in type 2 diabetics, increasing throughout the study
period. Archaea were significantly more frequent in type 2 diabetics.
Whether differences seen in the abundance of certain groups and in the diversity of microbiota reflect different
underlying inflammatory mechanisms of type 2 diabetes and obesity or rather the progression of the metabolic
disease remains unclear and needs long-term investigation.

Organisation(en)

Department für Ernährungswissenschaften

Externe Organisation(en)

Gesundheitszentrum Wien-Süd

Journal

Journal of Diabetes & Metabolism

Band

4

Seiten

1-8

Anzahl der Seiten

8

ISSN

2155-6156

DOI

https://doi.org/10.4172/2155-6156.1000253

Publikationsdatum

02-2013

Peer-reviewed

Ja

ÖFOS 2012

303009 Ernährungswissenschaften

Sustainable Development Goals

SDG 3 – Gesundheit und Wohlergehen

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/abundance-and-diversity-of-microbiota-in-type-2-diabetes-and-obesity(f73b6d29-15c6-49eb-b43d-fc10d9956cd5).html