Oral citrulline supplementation protects female mice from the development of non-alcoholic fatty liver disease (NAFLD)

Autor(en)
Cathrin Sellmann, Cheng Jun Jin, Anna Janina Engstler, Jean-Pascal De Bandt, Ina Bergheim
Abstrakt

PURPOSE: Impairments of intestinal barrier function are discussed as risk factors for the development and progression of non-alcoholic fatty liver disease (NAFLD). Studies suggest an association between arginine/citrulline homeostasis and the development of liver damages. Here, the effect of an oral L-citrulline (Cit) supplement on the development of a Western-style diet (WSD)-induced NAFLD was determined in mice.

METHODS: Female 6- to 8-week-old C57BL/6J mice were either pair-fed a liquid Western-style or control diet (C) ± 2.5 g/kg bodyweight Cit for 6 weeks (C + Cit or WSD + Cit). Indices of liver damage, glucose metabolism, intestinal barrier function and NO synthesis were measured.

RESULTS: While bodyweight gain was similar between groups, markers of glucose metabolism like fasting blood glucose and HOMA index and markers of liver damage like hepatic triglyceride levels, number of neutrophils and plasminogen activator inhibitor-1 protein levels were significantly lower in WSD + Cit-fed mice when compared to WSD-fed mice only. Protein levels of the tight junction proteins occludin and zonula occludens-1 in duodenum were significantly lower in mice fed a WSD when compared to those fed a WSD + Cit (-~70 and -~60 %, respectively, P < 0.05), whereas portal endotoxin levels, concentration of 3-nitrotyrosine protein adducts in duodenum and toll-like receptor-4 mRNA expression in livers of WSD + Cit-fed mice were markedly lower than in WSD-fed mice (-~43 %, P = 0.056; -~80 and -~48 %, respectively, P < 0.05).

CONCLUSION: Our data suggest that the protective effects of supplementing Cit on the development of NAFLD in mice are associated with a decreased translocation of endotoxin into the portal vein.

Organisation(en)
Externe Organisation(en)
Friedrich-Schiller-Universität Jena, Université Paris V - René Descartes, Paris Centre University Hospitals
Journal
European Journal of Nutrition
Band
56
Seiten
2519-2527
Anzahl der Seiten
9
ISSN
1436-6207
DOI
https://doi.org/10.1007/s00394-016-1287-9
Publikationsdatum
08-2016
Peer-reviewed
Ja
ÖFOS 2012
303009 Ernährungswissenschaften
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/oral-citrulline-supplementation-protects-female-mice-from-the-development-of-nonalcoholic-fatty-liver-disease-nafld(e35528c5-4023-4121-8e3b-3f2c6d1a1f07).html