A Xanthohumol-Rich Hop Extract Diminishes Endotoxin-Induced Activation of TLR4 Signaling in Human Peripheral Blood Mononuclear Cells: A Study in Healthy Women
- Autor(en)
- Finn Jung, Raphaela Staltner, Anja Baumann, Katharina Burger, Emina Halilbasic, Claus Hellerbrand, Ina Bergheim
- Abstrakt
Infections with Gram-negative bacteria are still among the leading causes of infection-related deaths. Several studies suggest that the chalcone xanthohumol (XN) found in hop (Humulus lupulus) possesses anti-inflammatory effects. In a single-blinded, placebo controlled randomized cross-over design study we assessed if the oral intake of a single low dose of 0.125 mg of a XN derived through a XN-rich hop extract (75% XN) affects lipopolysaccharide (LPS)-induced immune responses in peripheral blood mononuclear cells (PBMCs) ex vivo in normal weight healthy women (n = 9) (clinicaltrials.gov: NCT04847193) and determined associated molecular mechanisms. LPS-stimulation of PBMCs isolated from participants 1 h after the intake of the placebo for 2 h resulted in a significant induction of pro-inflammatory cytokine release which was significantly attenuated when participants had consumed XN. The XN-dependent attenuation of proinflammatory cytokine release was less pronounced 6 h after the LPS stimulation while the release of sCD14 was significantly reduced at this timepoint. The LPS-dependent activation of hTLR4 transfected HEK293 cells was significantly and dose-dependently suppressed by the XN-rich hop extract which was attenuated when cells were co-challenged with sCD14. Taken together, our results suggest even a one-time intake of low doses of XN consumed in a XN-rich hop extract can suppress LPS-dependent stimulation of PBMCs and that this is related to the interaction of the hop compound with the CD14/TLR4 signaling cascade.
- Organisation(en)
- Department für Ernährungswissenschaften
- Externe Organisation(en)
- Medizinische Universität Wien, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
- Journal
- International Journal of Molecular Sciences
- Band
- 23
- ISSN
- 1422-0067
- DOI
- https://doi.org/10.3390/ijms232012702
- Publikationsdatum
- 10-2022
- Peer-reviewed
- Ja
- ÖFOS 2012
- 303009 Ernährungswissenschaften
- Schlagwörter
- ASJC Scopus Sachgebiete
- Molecular Biology, Spectroscopy, Catalysis, Inorganic Chemistry, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry
- Sustainable Development Goals
- SDG 3 – Gesundheit und Wohlergehen
- Link zum Portal
- https://ucris.univie.ac.at/portal/de/publications/a-xanthohumolrich-hop-extract-diminishes-endotoxininduced-activation-of-tlr4-signaling-in-human-peripheral-blood-mononuclear-cells-a-study-in-healthy-women(7f0570a1-7cd9-4439-bd48-8116590db73e).html