Selenium-binding Protein 1 (SELENBP1) is a marker of mature adipocytes

Autor(en)
Holger Steinbrenner, Mustafa Micoogullari, Ngoc Anh Hoang, Ina Bergheim, Lars-Oliver Klotz, Helmut Sies
Abstrakt

Selenium-binding protein 1 (SELENBP1) has recently been reported to catalyse the oxidation of methanethiol, an organosulfur compound produced by gut microbiota. Two of the reaction products of methanethiol oxidation, hydrogen peroxide and hydrogen sulphide, serve as signalling molecules for cell differentiation. Indeed, colonocyte differentiation has been found to be associated with SELENBP1 induction. Here, we show that SELENBP1 is induced when 3T3-L1 preadipocytes undergo terminal differentiation and maturation to adipocytes. SELENBP1 induction succeeded the up-regulation of known marker proteins of white adipocytes and the intracellular accumulation of lipids. Immunofluorescence microscopy revealed predominant cytoplasmic localisation of SELENBP1 in 3T3-L1 adipocytes, as demonstrated by co-staining with the key lipogenic enzyme, acetylCoA-carboxylase (ACC), located in cytosol. In differentiating 3T3-L1 cells, the mTOR inhibitor rapamycin and the pro-inflammatory cytokine tumour necrosis factor alpha (TNF-alpha) likewise suppressed SELENBP1 induction, adipocyte differentiation and lipid accumulation. However, lipid accumulation per se is not linked to SELENBP1 induction, as hepatic SELENBP1 was down-regulated in high fructose-fed mice despite increased lipogenesis in the liver and development of non-alcoholic fatty liver disease (NAFLD). In conclusion, SELENBP1 is a marker of cell differentiation/maturation rather than being linked to lipogenesis/lipid accumulation.

Organisation(en)
Department für Ernährungswissenschaften
Externe Organisation(en)
Heinrich-Heine-Universität Düsseldorf, Friedrich-Schiller-Universität Jena, Leibniz-Institut für umweltmedizinische Forschung
Journal
Redox biology
Band
20
Seiten
489-495
Anzahl der Seiten
7
DOI
https://doi.org/10.1016/j.redox.2018.11.004
Publikationsdatum
01-2019
Peer-reviewed
Ja
ÖFOS 2012
303009 Ernährungswissenschaften
Schlagwörter
ASJC Scopus Sachgebiete
Clinical Biochemistry, Organic Chemistry
Link zum Portal
https://ucrisportal.univie.ac.at/de/publications/seleniumbinding-protein-1-selenbp1-is-a-marker-of-mature-adipocytes(96936936-4ac4-4136-863a-e68af0175fdf).html