Supplementing L-Citrulline Can Extend Lifespan in C. elegans and Attenuate the Development of Aging-Related Impairments of Glucose Tolerance and Intestinal Barrier in Mice
- Autor(en)
- Dragana Rajcic, Franziska Kromm, Angélica Hernández-Arriaga, Annette Brandt, Anja Baumann, Raphaela Staltner, Amélia Camarinha-Silva, Ina Bergheim
- Abstrakt
L-Citrulline (L-Cit) is discussed to possess a protective effect on intestinal barrier dysfunction but also to diminish aging-associated degenerative processes. Here, the effects of L-Cit on lifespan were assessed in C. elegans, while the effects of L-Cit on aging-associated decline were determined in C57BL/6J mice. For lifespan analysis, C. elegans were treated with ±5 mM L-Cit. Twelve-month-old male C57BL/6J mice (n = 8–10/group) fed a standard chow diet received drinking water ± 2.5 g/kg/d L-Cit or 5 g/kg/d hydrolyzed soy protein (Iso-N-control) for 16 or 32 weeks. Additionally, 4-month-old C57BL/6J mice were treated accordingly for 8 weeks. Markers of senescence, glucose tolerance, intestinal barrier function, and intestinal microbiota composition were analyzed in mice. L-Cit treatment significantly extended the lifespan of C. elegans. The significant increase in markers of senescence and signs of impaired glucose tolerance found in 16- and 20-month-old control mice was attenuated in L-Cit-fed mice, which was associated with protection from intestinal barrier dysfunction and a decrease in NO
2
− levels in the small intestine, while no marked differences in intestinal microbiota composition were found when comparing age-matched groups. Our results suggest that pharmacological doses of L-Cit may have beneficial effects on lifespan in C. elegans and aging-associated decline in mice.
- Organisation(en)
- Department für Ernährungswissenschaften
- Externe Organisation(en)
- Universität Hohenheim
- Journal
- Biomolecules
- Band
- 13
- DOI
- https://doi.org/10.3390/biom13111579
- Publikationsdatum
- 10-2023
- Peer-reviewed
- Ja
- ÖFOS 2012
- 303009 Ernährungswissenschaften
- Schlagwörter
- ASJC Scopus Sachgebiete
- Molecular Biology, Biochemistry
- Link zum Portal
- https://ucrisportal.univie.ac.at/de/publications/supplementing-lcitrulline-can-extend-lifespan-in-c-elegans-and-attenuate-the-development-of-agingrelated-impairments-of-glucose-tolerance-and-intestinal-barrier-in-mice(eb460da7-f4a4-444a-9e1a-c365e3c4db1b).html