Fractionation of an Extract of Pluchea odorata Separates a Property Indicative for the Induction of Cell Plasticity from One That Inhibits a Neoplastic Phenotype

Autor(en)

Mareike Seelinger, Ruxandra McKinnon, Benedikt Giessrigl, Christine Unger, Sabine Bauer, Karl-Heinz Wagner, Monika Fritzer-Szekeres, Thomas Szekeres, Rene Diaz, Foster M. Tut, Richard Frisch, Bjorn Feistel, Brigitte Kopp, Georg Krupitza

Abstrakt

Introduction. Several studies demonstrated that anti-inflammatory remedies exhibit excellent anti-neoplastic properties. An extract of Pluchea odorata (Asteraceae), which is used for wound healing and against inflammatory conditions, was fractionated and properties correlating to anti-neoplastic and wound healing effects were separated. Methods. Up to six fractionation steps using silica gel, Sephadex columns, and distinct solvent systems were used, and eluted fractions were analysed by thin layer chromatography, apoptosis, and proliferation assays. The expression of oncogenes and proteins regulating cell migration was investigated by immunoblotting after treating HL60 cells with the most active fractions. Results. Sequential fractionations enriched anti-neoplastic activities which suppressed oncogene expression of JunB, c-Jun, c-Myc, and Stat3. Furthermore, a fraction (F4.6.3) inducing or keeping up expression of the mobility markers MYPT, ROCK1, and paxillin could be separated from another fraction (F4.3.7), which inhibited these markers. Conclusions. Wound healing builds up scar or specific tissue, and hence, compounds enhancing cell migration support this process. In contrast, successful anti-neoplastic therapy combats tumour progression, and thus, suppression of cell migration is mandatory.

Organisation(en)

Department für Ernährungswissenschaften

Externe Organisation(en)

Medizinische Universität Wien, International Institute for Ethnobiological and Maya-Research and Ethnobotany, Finzelberg GmbH & Co KG

Journal

Evidence Based Complementary and Alternative Medicine

Band

2012

Seiten

701927

ISSN

1741-427X

DOI

https://doi.org/10.1155/2012/701927

Publikationsdatum

2012

Peer-reviewed

Ja

ÖFOS 2012

301204 Pharmakognosie

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/fractionation-of-an-extract-of-pluchea-odorata-separates-a-property-indicative-for-the-induction-of-cell-plasticity-from-one-that-inhibits-a-neoplastic-phenotype(05580328-e42a-4180-835a-fc0d1cc47a55).html