Extracellular and intracellular anti-mutagenic effects of bile pigments in the Salmonella typhimurium reverse mutation assay

Autor(en)

Christine Mölzer, Katharina Schubert, Andrew Cameron Bulmer, Karl-Heinz Wagner

Abstrakt

In vitro anti-genotoxic properties of bile pigments have been explored and confirmed recently. Despite these reports mechanisms to explain DNA protection by endogenous bile pigments remain unclear. Surprisingly, the quantification of cellular pigment absorption which could represent a fundamental prerequisite for intracellular (e.g., anti-mutagenic) effects, has not been explored. Therefore, we aimed to measure the amounts of un-/conjugated bilirubin as well as biliverdin absorbed into colonies of Salmonella typhimurium, utilising HPLC analyses, and to observe whether intracellular compound concentrations could predict anti-genotoxic effects. HPLC analyses confirmed that bacterial bile pigment absorption was concentration-dependent. Plate bile pigment concentrations were inversely associated with genotoxicity of all tested mutagens, irrespective of strain and test conditions. However, protection against frame-shift mutation in strain TA98 most strongly depended on the bacterial absorption of bilirubin and biliverdin, which indicates that bile pigments can protect by intercepting mutations extracellularly and specifically inhibit frame-shift mutations intracellularly.

Organisation(en)

Department für Ernährungswissenschaften

Externe Organisation(en)

Griffith University

Journal

Toxicology in Vitro

Band

27

Seiten

433-437

Anzahl der Seiten

5

ISSN

0887-2333

DOI

https://doi.org/10.1016/j.tiv.2012.08.004

Publikationsdatum

2013

Peer-reviewed

Ja

ÖFOS 2012

303009 Ernährungswissenschaften

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/bc4f60b5-610e-411f-9b95-e712170e4084