DNA damage and oxidative stress biomarkers in healthy male volunteers after a repeated bolus and continuous glucose infusion
- Autor(en)
- Laura Bragagna, Christina Polak, Lisa Schütz, Lina Maqboul, Carmen Klammer, Roland Feldbauer, Agnes Draxler, Martin Clodi, Karl-Heinz Wagner
- Abstrakt
Glucose variability (GV) is a phenomenon that describes fluctuations in blood glucose levels over the course of a day. As part of the investigation of risk factors for coronary heart disease, GV is increasingly becoming the focus of scientific attention. Scattering GV can contribute to the development of metabolic syndrome and type 2 diabetes. Hyperglycemia can lead to oxidative stress, which results in molecular damage due to an accumulation of reactive oxygen species (ROS). To obtain more information on the immediate effects of GV, 10 healthy men aged 21–30 years were administered intravenous glucose continuously or as a repeated bolus over a 48-hour period in a crossover study design.Whole blood was analysed for DNA damage using the Comet assay using three different incubation solutions (lysis buffer, H2O2 and the lesion-specific enzyme formamidopyrimidine DNA glycosylase (FPG)) and plasma for various markers of oxidative stress (protein carbonyls (PC), unconjugated bilirubin (UCB) and total antioxidant potential (FRAP). A significant time effect was found for the three incubation solutions for DNA damage, PC and UCB. This can potentially be attributed to circadian changes. However, no differences were observed for any of the markers between the two intervention groups. In conclusion, it was shown that bolus glucose administration had no significant acute effect on DNA damage and oxidative stress markers in healthy men compared to continuous administration.
- Organisation(en)
- Department für Ernährungswissenschaften
- Externe Organisation(en)
- Universität Wien, Krankenhaus der Barmherzigen Brüder Linz, Johannes Kepler Universität Linz
- Band
- 75
- Seiten
- 117-117
- Publikationsdatum
- 2025
- Peer-reviewed
- Ja
- ÖFOS 2012
- 303009 Ernährungswissenschaften, 301303 Medizinische Biochemie, 301110 Physiologie
- Sustainable Development Goals
- SDG 3 – Gesundheit und Wohlergehen
- Link zum Portal
- https://ucrisportal.univie.ac.at/de/publications/1baa0502-8924-47d8-8875-2bc016012611