Influence of age and physical fitness on miRNA-21, TGF-β and its receptors in leukocytes of healthy women

Autor(en)

Barbara Halper, Marlene Hofmann, Stefan Oesen, Bernhard Franzke, Petra Stuparits, Claudia Vidotto, Harald Tschan, Norbert Bachl, Eva-Maria Strasser, Michael Quittan, Karl-Heinz Wagner, Barbara Wessner

Abstrakt

The TGF-β superfamily has been shown to play an important role in a wide

range of physiological as well as pathological processes including

ageing, immune modulation, atherosclerosis and cancer development. The

aim of the current study was to investigate (i) whether TGF-β signalling

in peripheral blood mononuclear cells (PBMCs) would differ between

young and old females and (ii) whether physical performance parameters

of elderly women would be related to the expression of TGF-β or its

receptors. Sixteen healthy young (22-28 years; YF) and 90 healthy older

(65-92 years; OF) females participated in the study. In addition to

several components of health-related physical fitness, circulating CRP

and TGF-β levels were determined together with the mRNA expression of

TGF-β, TGF-βRI, TGF-βRII, and miRNA-21 (known to interfere with TGF-β

signalling) in PBMCs. Physical fitness as determined by 6-minutes

walking test (YF:median 932 (range 573-1254) m; OF:360 (114-558) m),

handgrip strength (YF: 32 (24-39) kg; OF:18(10-30) kg), relative

isokinetic peak torque of knee extensors (YF:1.9 (1.2- 2.3) Nm/kg;

OF:1.0 (0.2-1.9) Nm/kg and flexors (YF: 1.1 (0.7- 1.5) Nm/kg; OF: 0.5

(0.2-1.0) Nm/kg was substantially lower in older women (p<0.001 for

all comparisons). These changes were paralleled by an increase in hs-CRP

(YF: 0.9 (0.1-4.3)mg/L; OF: 2.3 (0.3-56.7)mg/L,p<0.001). Serum

levels of TGF-β and TGF-β mRNA levels from PBMCs did not differ between

young and old women whereas, both TGF- βRI/GAPDH (YF: 4.07 (1.38-14.60);

OF: 2.08 (0.14-28.81); p=0.020) and TGF-βRII/GAPDH levels (YF: 3.16

(1.14- 10.25); OF: 1.71 (0.51-14.86); p=0.020) were lower with respect

to old age. In elderly women, only TGF-βRΙ expression correlated

negatively with miRNA-21 expression in PBMCs (ρ=-0.315; p=0.004).

Interestingly, hs-CRP and miRNA correlated positively with handgrip

strength (ρ=0.237 and ρ=243, p<0.05), while none of the TGF-β-related

parameters were related to physical performance. The results suggest

that age affects TGF-β signalling in leukocytes by altering the

expression levels of its receptors. These changes seem to occur

independently of physical fitness of old women.

Organisation(en)

Institut für Sport- und Bewegungswissenschaft, Department für Ernährungswissenschaften

Externe Organisation(en)

Study Lab GmbH, Sozialmedizinisches Zentrum Süd – Kaiser-Franz-Josef-Spital

Journal

Exercise Immunology Review

Band

Seiten

154-163

Anzahl der Seiten

ISSN

1077-5552

Publikationsdatum

2015

Peer-reviewed

ÖFOS 2012

303028 Sportwissenschaft, 106023 Molekularbiologie, 305908 Sportmedizin

Schlagwörter

ASJC Scopus Sachgebiete

Immunology

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/221de050-9be8-4e77-8004-30d2ecefa9b0