Circulating cell-free DNA, telomere length and bilirubin in the Vienna Active Ageing Study: exploratory analysis of a randomized, controlled trial

Autor(en)

Anela Tosevska, Bernhard Franzke, Marlene Hofmann, Immina Vierheilig, Barbara Schober-Halper, Stefan Oesen, Oliver Neubauer, Barbara Wessner, Karl-Heinz Wagner

Abstrakt

Telomere length (TL) in blood cells is widely used in human studies as a molecular marker of ageing. Circulating cell-free DNA (cfDNA) as well as unconjugated bilirubin (UCB) are dynamic blood constituents whose involvement in age-associated diseases is largely unexplored. To our knowledge, there are no published studies integrating all three parameters, especially in individuals of advanced age. Here we present a secondary analysis from the Vienna Active Aging Study (VAAS), a randomized controlled intervention trial in institutionalized elderly individuals (n = 101). Using an exploratory approach we combine three blood-based molecular markers (TL, UCB and cfDNA) with a range of primary and secondary outcomes from the intervention. We further look at the changes occurring in these parameters after 6-month resistance exercise training with or without supplementation. A correlation between UCB and TL was evident at baseline (p < 0.05), and both were associated with increased chromosomal anomalies such as nucleoplasmatic bridges and nuclear buds (p < 0.05). Of the three main markers explored in this paper, only cfDNA decreased significantly (p < 0.05) after 6-month training and dietary intervention. No clear relationship could be established between cfDNA and either UCB or TL. The trial was registered at ClinicalTrials.gov (NCT01775111).

Organisation(en)

Forschungsplattform Active Ageing, Institut für Sport- und Bewegungswissenschaft, Department für Ernährungswissenschaften

Journal

Scientific Reports

Band

6

Anzahl der Seiten

10

ISSN

2045-2322

DOI

https://doi.org/10.1038/srep38084

Publikationsdatum

12-2016

Peer-reviewed

Ja

ÖFOS 2012

303028 Sportwissenschaft, 106023 Molekularbiologie, 303009 Ernährungswissenschaften, 302020 Gerontologie

Schlagwörter

ASJC Scopus Sachgebiete

General

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/2aa2a948-0510-45b7-83d5-da73e28cc819