Anti-leukaemic effects of two extract types of Lactuca sativa correlate with the activation of Chk2, induction of p21, downregulation of cyclin D1 and acetylation of alpha-tubulin
- Autor(en)
- Manuela Gridling, Ruxandra McKinnon, Brigitte Kopp, Karl-Heinz Wagner, Liselotte Krenn, Georg Krupitza
- Abstrakt
The water extract of the lettuce Lactuca sativa, but not the ethyl acetate extract, inhibited the growth of HL-60 leukaemia cells and MCF-7 breast cancer cells. This correlated with the activation of checkpoint kinase 2 (Chk2). the induction of the tumour suppressor p21, and the severe downregulation of the proto-oncogene cyclin D1. The ethyl acetate extract, but not the water extract, induced HL-60 cell death, which correlated With the aectylation of alpha-tubulin. The acetylation of alpha-tubulin is indicative for microtubuli stabilisation such as induced by taxol. The calculated amount for human intake would require approximately 3 kg lettuce to reach the required concentration shown to inhibit 50% HL-60 proliferation.
- Organisation(en)
- Department für Ernährungswissenschaften
- Externe Organisation(en)
- Medizinische Universität Wien
- Journal
- Oncology Reports
- Band
- 23
- Seiten
- 1145-1151
- Anzahl der Seiten
- 7
- ISSN
- 1021-335X
- DOI
- https://doi.org/10.3892/or_00000744
- Publikationsdatum
- 2010
- Peer-reviewed
- Ja
- ÖFOS 2012
- 301204 Pharmakognosie
- Sustainable Development Goals
- SDG 3 – Gesundheit und Wohlergehen
- Link zum Portal
- https://ucris.univie.ac.at/portal/de/publications/antileukaemic-effects-of-two-extract-types-of-lactuca-sativa-correlate-with-the-activation-of-chk2-induction-of-p21-downregulation-of-cyclin-d1-and-acetylation-of-alphatubulin(3027c0cb-8d9a-4886-90bf-26774b2b7f44).html