Separation and isolation of β-sitosterol oxides and their non-mutagenic potential in the Salmonella microsome assay
- Autor(en)
- Karin Koschutnig, Suvi Kemmo, Anna Maija Lampi, Vieno Piironen, Cornelia Fritz-Ton, Karl Heinz Wagner
- Abstrakt
The recent increase in phytosterol-enriched functional food provokes questions concerning the safety of their oxidation products. However, most of the existing toxicity studies have been performed with mixtures instead of single compounds, a consequence of the lack of pure phytosterol oxidation product (POP)-standards. The objectives of this study were to take in use a method for the isolation of β-sitosterol oxidation products and to assess their mutagenic and pro-oxidative potential. Oxides were prepared by thermo-oxidation, purified by column chromatography and separated by a NP-HPLC-UV system. 7-ketositosterol, 7β-OH-sitosterol, 7α-OH-sitosterol, a mixture of 6β-OH-3-ketositosterol/6α-OH-3-ketositosterol (ratio 4:3) and a mixture of polar oxides were fractionated. Yields in the range of several milligrams per fraction were achieved within one HPLC-run. Identification and quantification was done by GC-MS and GC-FID, respectively. In the Ames test the collected fractions failed to show a mutagenic activity towards Salmonella typhimurium strains TA98, TA100 and TA102.
- Organisation(en)
- Department für Ernährungswissenschaften
- Externe Organisation(en)
- University of Helsinki
- Journal
- Food Chemistry
- Band
- 118
- Seiten
- 133-140
- Anzahl der Seiten
- 8
- ISSN
- 0308-8146
- DOI
- https://doi.org/10.1016/j.foodchem.2009.04.062
- Publikationsdatum
- 01-2010
- Peer-reviewed
- Ja
- ÖFOS 2012
- 303009 Ernährungswissenschaften
- Schlagwörter
- ASJC Scopus Sachgebiete
- Analytical Chemistry, Food Science
- Link zum Portal
- https://ucrisportal.univie.ac.at/de/publications/separation-and-isolation-of-sitosterol-oxides-and-their-nonmutagenic-potential-in-the-salmonella-microsome-assay(6e8022bc-13dc-47b2-a6a9-1e5538b7643c).html