Time course-dependent changes in the transcriptome of human skeletal muscle during recovery from endurance exercise

Autor(en)

Oliver Neubauer, Surendran Sabapathy, Lazarus Ross, Kevin Ashton, Barbara Wessner, Ben Desbrow, David Cameron-Smith, Karl-Heinz Wagner, Luke Haseler, Andrew Cameron Bulmer

Abstrakt

Reprogramming of gene expression is fundamental for skeletal muscle adaptations in response to endurance exercise. This study investigated the time course-dependent changes in the muscular transcriptome after an endurance exercise trial consisting of 1 h of intense cycling immediately followed by 1 h of intense running. Skeletal muscle samples were taken at baseline, 3 h, 48 h, and 96 h postexercise from eight healthy, endurance-trained men. RNA was extracted from muscle. Differential gene expression was evaluated using Illumina microarrays and validated with qPCR. Gene set enrichment analysis identified enriched molecular signatures chosen from the Molecular Signatures Database. Three hours postexercise, 102 gene sets were upregulated [family wise error rate (FWER), P < 0.05], including groups of genes related with leukocyte migration, immune and chaperone activation, and cyclic AMP responsive element binding protein (CREB) 1 signaling. Forty-eight hours postexercise, among 19 enriched gene sets (FWER, P<0.05), two gene sets related to actin cytoskeleton remodeling were upregulated. Ninety-six hours postexercise, 83 gene sets were enriched (FWER, P < 0.05), 80 of which were upregulated, including gene groups related to chemokine signaling, cell stress management, and extracellular matrix remodeling. These data provide comprehensive insights into the molecular pathways involved in acute stress, recovery, and adaptive muscular responses to endurance exercise. The novel 96 h postexercise transcriptome indicates substantial transcriptional activity potentially associated with the prolonged presence of leukocytes in the muscles. This suggests that muscular recovery, from a transcriptional perspective, is incomplete 96 h after endurance exercise involving muscle damage.

Organisation(en)

Department für Ernährungswissenschaften, Institut für Sport- und Bewegungswissenschaft

Externe Organisation(en)

Griffith University, Baker IDI Heart and Diabetes Institute, Bond University, University of Auckland

Journal

Journal of Applied Physiology

Band

116

Seiten

274-287

Anzahl der Seiten

14

ISSN

8750-7587

DOI

https://doi.org/10.1152/japplphysiol.00909.2013

Publikationsdatum

12-2013

Peer-reviewed

Ja

ÖFOS 2012

106023 Molekularbiologie, 305908 Sportmedizin, 301110 Physiologie

Schlagwörter

ASJC Scopus Sachgebiete

Physiology (medical), Physiology

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/time-coursedependent-changes-in-the-transcriptome-of-human-skeletal-muscle-during-recovery-from-endurance-exercise(9e435631-0956-44d7-a996-a5205e73cd27).html