Potent protection of gallic acid against DNA oxidation

Autor(en)

Franziska Ferk, Asima Chakraborty, Walter Jäger, Michael Kundi, Julia Bichler, Miroslav Mišík, Karl-Heinz Wagner, Bettina Grasl-Kraupp, Sandra Sagmeister, Gerald Haidinger, Christine Hoelzl, Armen Nersesyan, Maria Dušinská, Tatjana Simić, Siegfried Knasmüller

Abstrakt

Gallic acid (3,4,5-trihydroxybenzoic acid, GA) is a constituent of plant derived foods, beverages and herbal remedies. We investigated its DNA protective properties in a placebo controlled human intervention trial in single cell gel electrophoresis experiments. Supplementation of drinking water with GA (12.8 mg/person/d) for three days led to a significant reduction of DNA migration attributable to oxidised pyrimidines (endonuclease III sensitive sites) and oxidised purines (formamidopyrimidine glycosylase sensitive sites) in lymphocytes of healthy individuals by 75% and 64% respectively. Also DNA damage caused by treatment of the cells with reactive oxygen species (ROS) was reduced after GA consumption (by 41%). These effects were paralleled by an increase of the activities of antioxidant enzymes (superoxide dismutase, glutathione peroxidase and glutathion-S-transferase-pi) and a decrease of intracellular ROS concentrations in lymphocytes, while no alterations of the total antioxidant capacity (TAC), of malondialdehyde levels in serum and of the urinary excretion of isoprostanes were found. Experiments with rats showed that GA reduces oxidatively damaged DNA in lymphocytes, liver, colon and lungs and protects these organs against gamma-irradiation-induced strand breaks and formation of oxidatively damaged DNA-bases. Furthermore, the number of radiation-induced preneoplastic hepatic foci was decreased by 43% after oral administration of the phenolic. Since we did not find alterations of the TAC in plasma and lipid peroxidation of cell membranes but intracellular effects it is likely that the antioxidant properties of GA seen in vivo are not due to direct scavenging of radicals but rather to indirect mechanisms (e.g. protection against ROS via activation of transcription factors). As the amount of GA used in the intervention trial is similar to the daily intake in Middle Europe (18 mg/person/day), our findings indicate that it may contribute to prevention of formation of oxidatively damaged DNA in humans.

Organisation(en)

Institut für Psychologie der Kognition, Emotion und Methoden, Department für Ernährungswissenschaften, Forschungsgruppe Workflow Systems and Technology

Externe Organisation(en)

Medizinische Universität Wien, Norwegian Institute for Air Research

Journal

Mutation Research: fundamental and molecular mechanisms of mutagenesis

Band

715

Seiten

61-71

Anzahl der Seiten

11

ISSN

1386-1964

DOI

https://doi.org/10.1016/j.mrfmmm.2011.07.010

Publikationsdatum

10-2011

Peer-reviewed

Ja

ÖFOS 2012

301209 Pharmazie

Schlagwörter

Sustainable Development Goals

SDG 3 – Gesundheit und Wohlergehen

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/potent-protection-of-gallic-acid-against-dna-oxidation(b45970fb-14b1-4083-a639-78fd0b3068ed).html