The anti-mutagenic and antioxidant effects of bile pigments in the Ames Salmonella test

Autor(en)

A. C. Bulmer, K. Ried, J. S. Coombes, J. T. Blanchfield, I. Toth, K. H. Wagner

Abstrakt

The aim of this study was to explore the potential pro- and anti-mutagenic effects of endogenous bile pigments unconjugated bilirubin (BR), biliverdin (BV) and a synthetic, water soluble conjugate, bilirubin ditaurate (BRT) in the Ames Salmonella test. The bile pigments were tested over a wide concentration range (0.01-2 μmol/plate) in the presence of three bacterial strains (TA98, TA100, TA102). A variety of mutagens including benzo[α]pyrene (B[α]P), 2,4,7 trinitrofluorenone (TNFone), 2-aminofluorene (2-AF), sodium azide (NaN₃) and tertiary-butyl hydroperoxide (t-BuOOH), were used to promote the formation of mutant revertants. Tests were conducted with (B[α]P, 2-AF, t-BuOOH) and without (TNFone, NaN₃, t-BuOOH) metabolic activation incorporating the addition of the microsomal liver preparation, S9. The bile pigments alone did not induce mutagenicity in any of the strains tested (p > 0.05). Anti-mutagenic effects of the bile pigments were observed in the presence of all mutagens except for NaN₃ and the anti-mutagenic effects appeared independent of the strain tested. For TNFone induced genotoxicity, the order of effectiveness was BR ≥ BRT > BV. However, the order was BV ≥ BRT ≥ BR for 2-AF. Antioxidant testing in the TA102 strain revealed bile pigments could effectively inhibit the genotoxic effect of t-BuOOH induced oxidative stress. The apparent antioxidant and anti-mutagenic behaviour of bile pigments further suggests their presence in biological systems is of possible physiological importance.

Organisation(en)

Department für Ernährungswissenschaften

Externe Organisation(en)

Queensland University of Technology, Biopharma Pty. Ltd., Universität Wien

Journal

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Band

629

Seiten

122-132

Anzahl der Seiten

11

ISSN

1383-5718

DOI

https://doi.org/10.1016/j.mrgentox.2007.01.008

Publikationsdatum

05-2007

Peer-reviewed

Ja

ÖFOS 2012

303009 Ernährungswissenschaften

Schlagwörter

ASJC Scopus Sachgebiete

Genetics, Health, Toxicology and Mutagenesis

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/the-antimutagenic-and-antioxidant-effects-of-bile-pigments-in-the-ames-salmonella-test(d9ee2d4f-a607-4671-97ee-705d417ce8b7).html