Biliverdin modulates the expression of C5aR in response to endotoxin in part via mTOR signaling

Autor(en)

Kavita Bisht, Barbara Wegiel, Jens Tampe, Oliver Neubauer, Karl-Heinz Wagner, Leo E Otterbein, Andrew C Bulmer

Abstrakt

Macrophages play a crucial role in the maintenance and resolution of inflammation and express a number of pro- and anti-inflammatory molecules in response to stressors. Among them, the complement receptor 5a (C5aR) plays an integral role in the development of inflammatory disorders. Biliverdin and bilirubin, products of heme catabolism, exert anti-inflammatory effects and inhibit complement activation. Here, we define the effects of biliverdin on C5aR expression in macrophages and the roles of Akt and mammalian target of rapamycin (mTOR) in these responses. Biliverdin administration inhibited lipopolysaccharide (LPS)-induced C5aR expression (without altering basal expression), an effect partially blocked by rapamycin, an inhibitor of mTOR signaling. Biliverdin also reduced LPS-dependent expression of the pro-inflammatory cytokines TNF-α and IL-6. Collectively, these data indicate that biliverdin regulates LPS-mediated expression of C5aR via the mTOR pathway, revealing an additional mechanism underlying biliverdin's anti-inflammatory effects.

Organisation(en)

Department für Ernährungswissenschaften

Externe Organisation(en)

Harvard University, Griffith University

Journal

Biochemical and Biophysical Research Communications

Band

449

Seiten

94-99

Anzahl der Seiten

6

ISSN

0006-291X

DOI

https://doi.org/10.1016/j.bbrc.2014.04.150

Publikationsdatum

06-2014

Peer-reviewed

Ja

ÖFOS 2012

303009 Ernährungswissenschaften

Schlagwörter

ASJC Scopus Sachgebiete

Molecular Biology, Biophysics, Biochemistry, Cell Biology

Link zum Portal

https://ucris.univie.ac.at/portal/de/publications/biliverdin-modulates-the-expression-of-c5ar-in-response-to-endotoxin-in-part-via-mtor-signaling(da9b0298-1511-4b57-9a80-47cb6ceea519).html