Effects of unconjugated bilirubin on chromosomal damage in individuals with Gilbert`s syndrome measured with the micronucleus cytome assay

Autor(en)

Marlies Wallner, Clemens Marischen, Maria-Theresia Pappenheim, Elisabeth Müllner, Christine Mölzer, Armen Nersesyan, Rodrig Marculescu, Daniel Doberer, Siegfried Knasmüller, Andrew Cameron Bulmer, Karl-Heinz Wagner

Abstrakt

Circulating unconjugated bilirubin (UCB) has been reported to protect against lung and colorectal cancer. The present study aimed to explore, for the first time, whether mildly elevated circulating UCB, as found in Gilbert`s syndrome (GS), is associated with changes of DNA damage. A random 76 individuals, matched for age and gender, were recruited from the general population and allocated into the GS group (UCB >= 17.1 mu M; n = 38) or control group (UCB <17.1 mu M; n = 38). Chromosomal and cytological changes were determined in lymphocytes and buccal cells using the cytokinesis-block micronucleus cytome assay (CBMN) and buccal micronucleus cytome assay (BMcyt). No significant differences were found between GS subjects and the control group in the CBMN and BMcyt determined endpoints. Subsequently, when age dependency of effects were analysed, lower formation of buccal micronucleated cells (by 73.3%) and buccal nuclear buds (by 70.9%) in the GS subgroup >= 30 years were found, compared to the GS subgroup <30 years. These findings suggest DNA protection in epithelial tissue of older individuals with GS.

Organisation(en)

Department für Ernährungswissenschaften, Institut für Publizistik- und Kommunikationswissenschaft

Externe Organisation(en)

Medizinische Universität Wien, Griffith University

Journal

Mutagenesis

Band

27

Seiten

731-735

Anzahl der Seiten

5

ISSN

0267-8357

DOI

https://doi.org/10.1093/mutage/ges039

Publikationsdatum

2012

Peer-reviewed

Ja

ÖFOS 2012

303009 Ernährungswissenschaften

Sustainable Development Goals

SDG 3 – Gesundheit und Wohlergehen

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/fa0b3ad7-4040-44ef-babf-39535df71fa5